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[Biochemistry](https://durgical.com/en/biochemistry/) · Molecule

# Fructose 1,6-bisphosphate

Fructose 1,6-bisphosphate is the two-phosphate sugar made by the committed step of glycolysis; aldolase splits it in two.

Updated 2026-10-31

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## Key facts

- Phosphofructokinase-1 makes it (step 3) using ATP; aldolase splits it into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate.
- It activates pyruvate kinase ahead of time: what comes in at the top speeds the exit at the bottom.
- In gluconeogenesis, fructose-1,6-bisphosphatase turns it back into fructose 6-phosphate; fructose 2,6-bisphosphate and AMP inhibit it.

**Also called**
F1,6BP

**Formula**
C₆H₁₄O₁₂P₂

**PubChem**
[CID 10267](https://pubchem.ncbi.nlm.nih.gov/compound/10267)

## Structure

- A furanose ring (five atoms) with phosphates on carbons 1 and 6; not to be confused with fructose 2,6-bisphosphate, which only regulates.

## Clinical notes

- Fructose-1,6-bisphosphatase deficiency causes fasting hypoglycemia and lactic acidosis, because it blocks gluconeogenesis.

## Pathways

- [Glycolysis](https://durgical.com/en/biochemistry/pathways/glycolysis/)

## Related conditions

- [Tarui disease (muscle phosphofructokinase deficiency)](https://durgical.com/en/conditions/tarui-disease/)

## Related

- [Phosphofructokinase-1 (PFK-1)](https://durgical.com/en/biochemistry/enzymes/phosphofructokinase-1/)
- [Pyruvate kinase](https://durgical.com/en/biochemistry/enzymes/pyruvate-kinase/)

## Test yourself

Think of the answer, then tap the question to see it.

## Which enzyme splits fructose 1,6-bisphosphate?

Aldolase, into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate.

## Which enzyme does fructose 1,6-bisphosphate activate ahead of time?

Pyruvate kinase (step 10).

## How does it differ from fructose 2,6-bisphosphate?

The 1,6 form is a glycolysis intermediate; the 2,6 form is not metabolised: it only activates PFK-1 and inhibits fructose-1,6-bisphosphatase.

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Molecules are shown in their neutral form, as the databases deposit them; in the cell the acids and phosphates are ionised.

Educational information, not medical advice. For any symptom, see a health professional.

Sources: [beta-D-Fructose 1,6-bisphosphate (CID 10267)](https://pubchem.ncbi.nlm.nih.gov/compound/10267) (PubChem, NCBI, 2026) · Public domain; [Glycolysis](https://reactome.org/PathwayBrowser/#/R-HSA-70171) (Reactome, 2026); Textbook of Biochemistry for Medical Students, 6th ed. (Jaypee Brothers Medical Publishers (Vasudevan DM, Sreekumari S, Vaidyanathan K), 2011).

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Representative model, for educational purposes only.

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Source: https://durgical.com/en/biochemistry/molecules/fructose-1-6-bisphosphate/
License: CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Cite as: DurgiPedia (durgical.com), https://durgical.com/en/biochemistry/molecules/fructose-1-6-bisphosphate/